rabbit anti- rat col iii  (Proteintech)

Journal: Journal of Cosmetic Dermatology

Article Title: ACE2 Inhibits Dermal Regeneration Through Ang II in Tissue Expansion

doi: 10.1111/jocd.16767

Figure Lengend Snippet: ACE2 inhibitor MLN‐4760 elevated the amount of collagens in the expanded rat skin. (a) TGF‐β mRNA expressions in DMSO‐treated and MLN‐760‐treated skins ( n = 3). (b) COL I mRNA expressions in DMSO‐treated and MLN‐760‐treated skins ( n = 3). (c) COL III mRNA expression in DMSO‐treated and MLN‐760‐treated skins ( n = 3). (d) Protein levels of TGF‐β, COL I, and COL III in DMSO‐treated and MLN‐760‐treated skins detected by Western blot. (e) Quantification of the TGF‐β protein levels in DMSO‐treated and MLN‐760‐treated skins ( n = 4). (f) Quantification of the COL I protein levels in DMSO‐treated and MLN‐760‐treated skins ( n = 4). (g) Quantification of the COL III protein levels in DMSO‐treated and MLN‐760‐treated skins ( n = 4). * p < 0.05, and ** p < 0.01.

Article Snippet: The membranes were immersed in a blocking solution for 1 h. The membranes were then immersed at least 12 h at 4°C °C with the subsequent primary antibodies: rabbit anti‐rat ACE2 (1:1000, Abcam, United Kingdom), rabbit anti‐rat COL I (1:2000, Proteintech, China), rabbit anti‐rat COL III (1:1000, Proteintech, China), and rabbit anti‐rat TGF‐β (1:1000, Proteintech, China).

Techniques: Expressing, Western Blot

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/IJN.S466312

Figure Lengend Snippet: RCDs/UA@Lipo alleviated H 2 O 2 -induced oxidative stress and TGF-β1 induced fibrosis-specific markers separately. ( A ) Immunofluorescence staining images of intracellular ROS and superoxide detected by DCFH-DA probe. ( B ) mitochondrial membrane potential (ΔΨm) detected by JC-1 probe. ( C ) tendon fibrosis markers (COL I, COL III, α-SMA) of NIH3T3. ( D–H ) Semiquantitative analysis of the relative fluorescent intensity of ( A–C ) (n = 3). ( D and E ) Model group is H 2 O 2 group, ( F–H ) Model group is TGF-β1 group. Data are presented as mean ± SD; comparisons between the groups were performed one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: The fresh tissue sections were incubated with rabbit anti-rat antibodies against COL III (1:200; Cat#22734-1-AP, Proteintech, USA) and α-SMA (1:200; Cat#19245S, Cell Signaling Technology, USA) at 4°C overnight.

Techniques: Immunofluorescence, Staining, Membrane

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/IJN.S466312

Figure Lengend Snippet: RCDs/UA@Lipo-HAMA reduced adhesion of injured tendons at macroscopic and histological levels at various time points post-injury. ( A ) Schematic diagram of surgical procedures of the ATI. ( B ) Gross view of ATI. ( C )Representative images of immunofluorescence staining of tendon antioxidant (Nrf-2, HO-1) and anti-inflammatory markers (CD68, iNOS) at 2 weeks post-injury (n = 3). ( D ) Representative images of immunofluorescence staining of tendon antifibrosis (COL III, α-SMA) at 6 weeks post-injury (n = 3). ( E ) Representative images of immunohistochemical staining of tendon markers (Vimentin, MMP2, α-SMA) antifibrosis at 6 weeks post-injury (n = 4). ( F–N ) Semiquantitative analysis of expression level of tendon marker of ( C–E ), respectively. Data are presented as mean ± SD; comparisons between the groups were performed by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: The fresh tissue sections were incubated with rabbit anti-rat antibodies against COL III (1:200; Cat#22734-1-AP, Proteintech, USA) and α-SMA (1:200; Cat#19245S, Cell Signaling Technology, USA) at 4°C overnight.

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Expressing, Marker